Publication

Published Articles




Yuko TomitaMasahiro Takeya, Kengo Suzuki, Nobuko Nitta, Chieko Higuchi, Yuka Marukawa-Hashimoto, Takashi Osanai. (2018) Amino acid excretion from Euglena gracilis cells in dark and anaerobic conditions. Algal Res. in press. doi.org/10.1016/j.algal.2018.11.017

Shoki Ito
Takashi Osanai
(2018) Single Amino Acid Change in 6-Phosphogluconate dehydrogenase from Synechocystis conveys higher affinity for NADP+ and Altered Mode of Inhibition by NADPH. Plant Cell Physiol.  doi: 10.1093/pcp/pcy165.
https://academic.oup.com/pcp/advance-article/doi/10.1093/pcp/pcy165/5069196

Noriaki Katayama
, Hiroko Iijima, Takashi Osanai. 
(2018) Production of Bioplastic Compounds by Genetically Manipulated and Metabolic Engineered Cyanobacteria.
In: Zhang W., Song X. (eds) Synthetic Biology of Cyanobacteria. Advances in Experimental Medicine and Biology, vol 1080. Springer, Singapore
https://link.springer.com/chapter/10.1007%2F978-981-13-0854-3_7

Masahiro Takeya
Shoki ItoHaruna Sukigara,
 Takashi Osanai.
(2018) Purification and Characterisation of Malate Dehydrogenase From Synechocystis sp. PCC 6803: Biochemical Barrier of the Oxidative Tricarboxylic Acid Cycle. Front. Plant Sci. 9:947. doi: 10.3389/fpls.2018.00947
https://doi.org/10.3389/fpls.2018.00947

Satomi Arisaka
Haruna Sukigara , Takashi Osanai 
(2018) Genetic manipulation to overexpress rpaA altered photosynthetic electron transport in Synechocystis sp. PCC 6803. J. Biosci. Bioeng. in press.
https://www.ncbi.nlm.nih.gov/pubmed/29519652

Shoki Ito
Masahiro TakeyaTakashi Osanai 
(2017) Substrate specificity and allosteric regulation of a D-lactate dehydrogenase from a unicellular cyanobacterium are altered by an amino acid substitution. Sci. Rep. 
https://www.ncbi.nlm.nih.gov/pubmed/29118438

Masahiro Takeya
Hiroko IijimaHaruna Sukigara,
 Takashi Osanai.
(2017) Cluster-level relationships of genes involved in carbon metabolism in Synechocystis sp. PCC 6803: Development of a novel succinate-producing strain. Plant Cell Physiol.
https://doi.org/10.1093/pcp/pcx162

Chika Yasuda
Hiroko IijimaHaruna SukigaraTakashi Osanai.
 (2017) Incubation of Cyanobacteria Under Dark, Anaerobic Conditions and Quantification of the Excreted Organic Acids by HPLC. Bio-protocol, 7(9): e2257. DOI: 10.21769/BioProtoc.2257.

Takashi Osanai
,
Ayuko Kuwahara, Hitomi Otuki, Kazuki Saito, Masami Yokota Hirai. (2017) ACR11 is an activator of plastid-type glutamine synthetase GS2 in Arabidopsis thaliana. Plant Cell Physiol.

Takashi Osanai
,
Youn-Il Park, Yuki Nakamura. (2017) Editorial: Biotechnology of microalgae, based on molecular biology and biochemistry of eukaryotic algae and cyanobacteria. Front. Microbiol.

Masahiro Takeya
Masami Yokota Hirai, Takashi Osanai.
(2017) Allosteric inhibition of phosphoenolpyruvate carboxylases is determined by a single amino acid residue in cyanobacteria. Sci. Rep. 7, 41080.

Yuko Tomita
Kazumasa YoshiokaHiroko Iijima
Ayaka Nakashima, Osamu Iwata, Kengo Suzuki, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai. (2016) Succinate and lacate production from Euglena gracilis during dark, anaerobic conditions. Front. Microbiol. doi: 10.3389/fmicb.2016.02050.

Sakiko Ueda*
Yuhki Kawamura*Hiroko IijimaMitsuharu Nakajima,
Tomokazu Shirai, Mami Okamoto, Akihiko Kondo, Masami Yokota Hirai,Takashi Osanai. (2016) Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria. Sci. Rep. 6:32354. *同等貢献

Hiroko Iijima
*
, Tomokazu Shirai*, Yuka Nakaya, Mami Okamoto, Filipe Pinto, Paula Tamagnini, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai (2016) Metabolomics-based analysis revealing the alteration of primary carbon metabolism by the genetic manipulation of a hydrogenase HoxH inSynechocystis sp. PCC 6803. Algal Research  18, 305-313. *同等貢献

Ayuko Kuwahara*Satomi Arisaka*Masahiro Takeya*, Hiroko Iijima, Masami Yokota Hirai, Takashi Osanai (2015) Modification of photosynthetic electron transport and amino acid levels by overexpression of a circadian-related histidine kinase hik8 inSynechocystis sp. PCC 6803. Front. Microbiol. 6:1150. *同等貢献

Takashi Osanai
, Tomokazu Shirai, Hiroko Iijima, Yuka Nakaya, Mami Okamoto, Akihiko Kondo, Masami Yokota Hirai. (2015) Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium. Front. Microbiol. 6: 1064.

Hiroko Iijima
,
 Tomokazu Shirai, Mami Okamoto, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai(2015) Changes in primary metabolism under light and dark conditions in response to overproduction of a response regulator RpaA in the unicellular cyanobacteriumSynechocystis sp. PCC 6803. 
Front. Microbiol. 6: 888.

Shirai T, Osanai T, Kondo A. (2016) Desinging intracellulcar metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechocystis sp. 6803 genome-scale model. Microb. Cell Fact. 15: 13

Hondo S, Takahashi M, Osanai T, Matsuda M, Hasunuma T, Tazuke A, Nakahira Y, Chohnan S, Hasegawa M, Asayama M. (2015) Genetic engineering and metabolic profiling for overproduction of polyhydroxybutyrate in cyanobacteria. J. Biosci. Bioeng. 120: 510-517.


Hiroko Iijima
, Yuka Nakaya, Ayuko Kuwahara, Masami Yokota Hirai, Takashi Osanai (2015) Seawater cultivation of freshwater cyanobacterium Synechocystis sp. PCC 6803 drastically alters amino acid composition and glycogen metabolism. Front. Microbiol. 6:362.


Yuka Nakaya*, Hiroko Iijima*, Junko Takanobu, Atsuko Watanabe, Masami Yokota, Hirai, Takashi Osanai (2015) One day of nitrogen starvatino reveals the effect of sigE and rre37 overexpression on the expression of genes related to carbon and nitrogen metabolism in Synechocystis sp PCC 6803. J. Biosci. Bioeng., doi: 10.1016/j.jbiosc.2014.12.020 (*Equally contributed)

Numata K, Motoda Y, Watanabe S, Osanai T, Kigawa T. (2015) Co-expression of two polyhydroxyalkanoate synthase subuntis from Synechocystis sp. PCC 6803 by cell-free synthesis and their specific activity for polymerization of 3-hydroxybutyryl-coenzyme A. Biochemistry 54: 1401-1407.


Takashi Osanai*, Tomokazu Shirai*, Hiroko Iijima, Ayuko Kuwahara, Iwane Suzuki, Akihiko Kondo and Masami Yokota Hirai. (2015) Alteration of cyanobacterial sugar and amino acid metabolism by overexpressino hik8, encoding a KaiC-associated histidine kinase. Environ. Microbiol. 17: 2430-2440 (*Equally contributed)

Hiroko Iijima, Atsuko Watanabe, Junko Takanobu, Masami Yokota, Hirai, Takashi Osanai (2014) rre37 overexpression alters gene expression related to the tricarboxylic acid cycle and pyruvate metabolism in Synechocystis sp PCC 6803. ScientificWorldJournal, 2014;2014:921976.

Takashi Osanai
*, Akira Oikawa*, Hiroko Iijima, Ayuko Kuwahara, Munehiko Asayama, Kan Tanaka, Masahiko Ikeuchi, Kazuki Saito, and Masami Yokota Hirai. (2014) Metabolomic Analysis Reveals Rewiring of Synechocystis sp. PCC 6803 Primary Metabolism by ntcA-overexpression. Environ. Microbiol. 16, 3304-3317 (*Equally contributed).


Takashi Osanai, Akira Oikawa, Keiji Numata, Ayuko Kuwahara, Hiroko Iijima, Yoshiharu Doi, Kazuki Saito, and Masami Yokota Hirai. (2014) Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803. Plant Physiol. 164, 1831-1841.

Takashi Osanai*, Akira Oikawa*, Tomokazu Shirai, Ayuko Kuwahara, Hiroko Iijima, Kan Tanaka, Masahiko Ikeuchi, Akihiko Kondo, Kazuki Saito, and Masami Yokota Hirai. (2014) Capillary electrophoresis-mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp. PCC 6803. Environ. Microbiol. 16, 512-524 (*Equally contributed).

Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Hiroshi Teramura, Tomohisa Hasunuma, Fumio Matsuda, Takashi Osanai, Masami Yokota Hirai, and Akihiko Kondo. (2014) Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803. FEBS Lett. 588, 466-471.

Takashi Osanai, Ayuko Kuwahara, Hiroko Iijima, Kiminori Toyooka, Mayuko Sato, Kan Tanaka, Masahiko Ikeuchi, Kazuki Saito, and Masami Yokota Hirai. (2013) Pleiotropic effect of sigE over-expression on cell morphology, photosynthesis and hydrogen production in Synechocystis sp. PCC 6803. Plant J. 76, 456-465.

Takashi Osanai, Keiji Numata, Akira Oikawa, Ayuko Kuwahara, Hiroko Iijima, Yoshiharu Doi, Kan Tanaka, Kazuki Saito, and Masami Yokota Hirai. (2013) Increased bioplastic production with an RNA polymerase sigma factor SigE during nitrogen starvation in Synechocystis sp. PCC 6803. DNA Res.20, 525-535.

Takashi Osanai#, Akira Oikawa, Miyuki Azuma, Kan Tanaka, Kazuki Saito, Masami Yokota Hirai, and Masahiko Ikeuchi. (2011) Genetic engineering of the group 2 sigma factor SigE widely activates the expressions of sugar catabolic genes in Synechocystis sp. PCC 6803. J. Biol. Chem. 286, 30962-30971. #Corresponding author

Miyuki Azuma*, Takashi Osanai*, Masami Yokota Hirai, and Kan Tanaka. (2011)A response regulator Rre37 and an RNA polymerase sigma factor SigE represent two parallel pathways to activate sugar catabolism in a cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 52, 404-412 (*Equally contributed).

Takashi Osanai#, Masahiko Imashimizu, Asako Seki, Shusei Sato, Satoshi Tabata, Sousuke Imamura, Munehiko Asayama, Masahiko Ikeuchi, and Kan Tanaka. (2009) ChlH, the H subunit of the Mg-chelatase, is an anti-sigma factor for SigE in Synechocystis sp. PCC 6803. Proc. Natl. Acad. Sci. USA 106, 6860-6865. #Corresponding author

Takashi Osanai, Masahiko Ikeuchi, and Kan Tanaka. (2008) Group 2 sigma factors in cyanobacteria. Physiol. Plant.133, 490-506. (Review)

Takashi Osanai, and Kan Tanaka. (2007) Keeping in touch with PII: PII-interacting proteins in unicellular cyanobacteria. Plant Cell Physiol.48, 908-914. (Review)

Takashi Osanai, Miyuki Azuma, and Kan Tanaka. (2007) Sugar catabolism regulated by light- and nitrogen-status in the cyanobacterium Synechocystis sp. PCC 6803. Photochem. Photobiol. Sci.6, 508-514. (Review)

Takashi Osanai, Sousuke Imamura, Munehiko Asayama, Makoto Shirai, Iwane Suzuki, Norio Murata, and Kan Tanaka. (2006) Nitrogen induction of sugar catabolic gene expression in Synechocystis sp. PCC 6803. DNA Res.13, 185-195.

Takashi Osanai, Shusei Sato, Satoshi Tabata, and Kan Tanaka. (2005) Identification of PamA as a PII-binding membrane protein important in nitrogen-related and sugar-catabolic gene expression in Synechocystis sp. PCC 6803. J. Biol. Chem. 280, 34684-34690.

Takashi Osanai, Yu Kanesaki, Takayuki Nakano, Hiroyuki Takahashi, Munehiko Asayama, Makoto Shirai, Minoru Kanehisa, Iwane Suzuki, Norio Murata, and Kan Tanaka. (2005) Positive regulation of sugar catabolic pathways in the cyanobacterium Synechocystis sp. PCC 6803 by the group 2 s factor SigE. J. Biol. Chem. 280, 30653-30659.

Munehiko Asayama, Sosuke Imamura, Satoshi Yoshihara, Ai Miyazaki, Naoko Yoshida, Takashi Sazuka, Takakazu Kaneko, Osamu Ohara, Satoshi Tabata, Takashi Osanai, Kan Tanaka, Hideo Takahashi, and Makoto Shirai. (2004) SigC, the Group 2 sigma factor of RNA polymerase, contributes to the late-stage gene expression and nitrogen promoter recognition in the cyanobacterium Synechocystis sp. strain PCC 6803. Biosci. Biotech. Biochem. 68, 477-487.