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Yoshimura, Hideyuki     Professor

Choi, Pak-Kon Professor
To the group homepage
http://www.isc.meiji.ac.jp/~biophys3/
Academic Degree
PhD (Science), Nagoya University, Japan 1982
Membership in Academic Societies
The Biophysical Society of Japan
The Japan Society of Applied Physics
The Japanese Society of Microscopy

Research Interests

Nanoparticles synthesized using ferritin
(A) Ferrite, (B) Al(OH)3, (C) Ca(OH)2, (D) Gold; scale bars represent 5 nm.
(i)
Development of an X-ray projection microscope
(ii)
Nanoparticle synthesis using proteins
(iii)
Structural and mechanical studies on bacterial flagella filaments

The aim of research in this laboratory is to investigate the structure and functions of biological materials and to utilize them for synthesis of innovative materials. We are currently pursuing three main themes directed at this aim. One of them is development of an X-ray microscope for biology. This microscope is an electron microscope based homemade apparatus that can be used to investigate the three-dimensional structure (X-ray micro-CT) of biological specimens. Another project addresses the synthesis of nanoparticles utilizing protein. Usually, we use the protein “ferritin”, which has a 7 nm diameter cavity inside that we utilize as a nano-scale reaction chamber. Ferritin accumulates metal ions into its cavity and mineralizes metal compounds as a nano-scale crystal (see figure). We are also investigating the mechanical properties of bacterial flagella, which have many characteristics that cannot be fabricated artificially.

Selected Publications

  1. H. Fukano, T. Takahashi, M. Aizawa, and H. Yoshimura, “Synthesis of Uniform and Dispersive Calcium Carbonate Nanoparticles in a Protein Cage through Control of Electrostatic Potential”, Inorganic Chemistry, 50, 6526-6532, (2011).
  2. H. Yoshimura, T. Ohigashi, M. Uesugi, K. Uesugi, T. Higashikawa, R. Nakamura, Y. Mori, K. Shinohara, “Sarcoptes scabiei var. hominis: Three-dimensional structure of a female imago and crusted scabies lesions by X-ray micro-CT”, Experimental Parasitology, 122, 268–272, (2009).
  3. H. Yoshimura, “Protein-assisted Nanoparticle synthesis”, Colloids and Surfaces A, 282-283, 464-470, (2006).
  4. M. Okuda, Y. Kobayashi, K. Suzuki, K. Sonoda, T. Kondoh, A. Wagawa, A. Kondo, and H. Yoshimura, “Self-Organized Inorganic Nanoparticle Arrays on Protein Lattices”, Nano Letters, 5, 991-993, (2005).
  5. “Two-dimensional crystallization”, N.D. Denkov, O.D. Velev, P.A. Kralchevsky, I.B. Ivanov, H. Yoshimura, and K. Nagayama, Nature, 361, 26, (1993).
  6. T. Akiba, H. Yoshimura and K. Namba, “Monolayer Crystallization of Flagellar L-P Rings by Sequential Addition and Depletion of Lipid”, Science, 252, 1544-1546 (1991).
  7. H. Yoshimura, M. Matsumoto, S. Endo and K. Nagayama, “Two-dimensional Crystallization of Proteins on Mercury”, Ultramicroscopy, 32, 265-274, (1990).
  8. H. Yoshimura, T. Nishio, K. Mihashi, K. Kinosita, Jr. and A. Ikegami, “Torsional Motion of Eosin-labeled F-actin as Detected in the Time-resolved Anisotropy Decay of the Probe in the Sub-millisecond Time Range”, J. Mol. Biol., 179, 453-467, (1984).
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